A weakly voltage-dependent, nonselective cation channel mediates toxic sodium influx in wheat

R. J. Davenport*, Mark Tester

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

173 Scopus citations

Abstract

To determine the transporters responsible for toxic Na+ influx in wheat (Triticum aestivum), root plasma membrane preparations were screened using the planar lipid bilayer technique as an assay for Na+-permeable ion channel activity. The predominant channel in the bilayer was a 44-pS channel that we called the nonselective cation (NSC) channel, which was nonselective for monovalent cations and weakly voltage dependent. Single channel characteristics of the NSC channel were compared with 22Na+ influx into excised root segments. Na+ influx through the NSC channel resembled 22Na+ influx in its partial sensitivity to inhibition by Ca2+, Mg2+, and Gd3+, and its insensitivity to all other inhibitors tested (tetraethylammonium, quinine, Cs+, tetrodotoxin, verapamil, amiloride, and flufenamate). Na+ influx through the NSC channel also closely resembled an instantaneous current in wheat root protoplasts (S.D. Tyerman, M. Skerrett, A. Garill, G.P. Findlay, R. Leigh [1997] J Exp Bot 48: 459-480) in its permeability sequence, selectivity for K+ over Na+ (approximately 1.25), insensitivity to tetraethylammonium, voltage independence, and partial sensitivity to Ca2+. Comparison of tissue, protoplast (S.D. Tyerman, M. Skerrett, A. Garill, G.P. Findlay, R. Leigh [1997] J Exp Bot 48: 459-480), and single-channel data indicate that toxic Na+ influx is catalyzed by a single transporter, and this is likely to be the NSC channel identified in planar lipid bilayers.

Original languageEnglish (US)
Pages (from-to)823-834
Number of pages12
JournalPlant physiology
Volume122
Issue number3
DOIs
StatePublished - Jan 1 2000

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science

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