A study of the incubation of microbead agglutination assays in a microfluidic system

David Castro, David Conchouso Gonzalez, Armando Arpys Arevalo Carreno, Ian G. Foulds

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations

Abstract

This work reports on a quantitative study of the incubation of a microbead-based agglutination assay inside a microfluidic system. In this system, a droplet (1.25µL) consisting of a mixture of functionalized microbeads and analyte is flowed through a 0.51mm internal diameter silicone tube. Hydrodynamic forces alone produce a very efficient mixing of the beads within the droplet. We tested the agglutination at different speeds and show a robust response at the higher range of speeds (150 – 200µL/min), while also reaching a completion in the agglutination process. At these velocities, a length of 180cm is shown to be sufficient to confidently measure the agglutination assay, which takes between 2.5 – 3 minutes. This high throughput quantification method has the potential of accelerating the measurements of various types of biomarkers, which can greatly benefit the fields of biology and medicine.
Original languageEnglish (US)
Title of host publication2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems (NEMS)
PublisherInstitute of Electrical and Electronics Engineers (IEEE)
Pages354-357
Number of pages4
ISBN (Print)9781509019472
DOIs
StatePublished - Dec 19 2016

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