A simple system for in-droplet incubation and quantification of agglutination assays

Daniela Castro, Rimantas Kodzius, Ian Grant Foulds

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

4 Scopus citations

Abstract

This work reports on a simple system for quantitative sensing of a target analyte based on agglutination in microchannels. Functionalized microbeads and analyte with no prior incubation are flowed in droplets (∼2μL) through a thin silicone tube filled with mineral oil at a flow rate of 150 μL/min. Hydrodynamic forces alone produce a highly efficient mixing of the beads within the droplet, without the need of complex mixing structures or magnetic actuation. The setup allows rapid observation of agglutination (<2 min), which is quantified using image analysis, and has potential application to high-throughput analysis.

Original languageEnglish (US)
Title of host publication17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
PublisherChemical and Biological Microsystems Society
ISBN (Print)9781632666246
StatePublished - Jan 1 2013

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