This work reports on a simple system for quantitative sensing of a target analyte based on agglutination in microchannels. Functionalized microbeads and analyte with no prior incubation are flowed in droplets (∼2μL) through a thin silicone tube filled with mineral oil at a flow rate of 150 μL/min. Hydrodynamic forces alone produce a highly efficient mixing of the beads within the droplet, without the need of complex mixing structures or magnetic actuation. The setup allows rapid observation of agglutination (<2 min), which is quantified using image analysis, and has potential application to high-throughput analysis.
|Original language||English (US)|
|Title of host publication||17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013|
|Publisher||Chemical and Biological Microsystems Society|
|State||Published - Jan 1 2013|