A simple method for isolation of megabase DNA from cotton

Xinping Zhao, Hong Bin Zhang, Rod A. Wing, Andrew H. Paterson

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

A simple method for preparation of high-molecular-weight DNA from cotton was developed. This method includes two major steps, (i) isolating nuclei and (ii) embedding nuclei into agarose microbeads. DNA isolated by this procedure is larger than 5.7 Mb in size, and is suitable for physical mapping by PFGE and YAC/BAC cloning. © 1994 Kluwer Academic Publishers.
Original languageEnglish (US)
JournalPlant Molecular Biology Reporter
Volume12
Issue number2
DOIs
StatePublished - Jun 1 1994
Externally publishedYes

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