A protoplast-based bioassay to quantify strigolactone activity in arabidopsis using strigoquant

Justine Braguy, Sophia L. Samodelov, Jennifer Andres, Rocio Ochoa-Fernandez, Salim Al-Babili, Matias D. Zurbriggen

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Understanding the biological background of strigolactone (SL) structural diversity and the SL signaling pathway at molecular level requires quantitative and sensitive tools that precisely determine SL dynamics. Such biosensors may be also very helpful in screening for SL analogs and mimics with defined biological functions. Recently, the genetically encoded, ratiometric sensor StrigoQuant was developed and allowed the quantification of the activity of a wide concentration range of SLs. StrigoQuant can be used for studies on the biosynthesis, function and signal transduction of this hormone class. Here, we provide a comprehensive protocol for establishing the use of StrigoQuant in Arabidopsis protoplasts. We first describe the generation and transformation of the protoplasts with StrigoQuant and detail the application of the synthetic SL analogue GR24. We then show the recording of the luminescence signal and how the obtained data are processed and used to assess/determine SL perception.
Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherSpringer US
Pages201-218
Number of pages18
ISBN (Print)9781071614280
DOIs
StatePublished - May 25 2021

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