A prevalent neglect of environmental control in mammalian cell culture calls for best practices

Shannon Klein, Samhan M. Alsolami, Alexandra Steckbauer, Silvia Arossa, Anieka Parry, Gerardo Ramos Mandujano, Khaled Alsayegh, Juan Carlos Izpisua Belmonte, Mo Li, Carlos M. Duarte

Research output: Contribution to journalArticlepeer-review

Abstract

Human cell lines, first cultured in the 1950s1, are indispensable in biomedical research. Today, a wide range of cell types are available, and sophisticated advanced ‘omics’ and visualization techniques allow for the routine assessment of cell identity and cellular responses2. However, the culture methods have remained relatively unchanged. Major advances in culture systems were made over three decades ago3,4, yet the old standard approach of batch cell culture — the culture of cells either in suspension or as adherent monolayers of cells in standard media5,6,7 — remains the predominant method in biomedical research.
Original languageEnglish (US)
JournalNature Biomedical Engineering
DOIs
StatePublished - Aug 13 2021

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