A novel non-diffractive reconstruction method for digital holographic microscopy

Michael Liebling, Thierry Blu, Etienne Cuche, Pierre Marquet, Christian Depeursinge, Michael Unser

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

28 Scopus citations

Abstract

We present a new method for reconstructing digitally recorded off-axis Fresnel holograms. Currently-used reconstruction methods are based on the simulation and propagation of a reference wave that is diffracted by the hologram. This procedure introduces a twin-image and a zero-order term which are inherent to the diffraction phenomenon. These terms perturb the reconstruction and limit the field of view. Our new approach splits the reconstruction process into two parts. First, we recover the amplitude and the phase in the camera plane from the measured hologram intensity. Our algorithm is based on the hypothesis of a slowly varying object wave which interferes with a more rapidly varying reference wave. In a second step, we propagate this complex wave to refocus it using the Fresnel transform. We therefore avoid the presence of the twin-image and zero-order interference terms. This new approach is flexible and can be adapted easily to complicated experimental setups. We demonstrate its feasibility in the case of digital holographic microscopy and present results for the imaging of living neurons.

Original languageEnglish (US)
Title of host publication2002 IEEE International Symposium on Biomedical Imaging, ISBI 2002 - Proceedings
PublisherIEEE Computer Society
Pages625-628
Number of pages4
ISBN (Electronic)078037584X
DOIs
StatePublished - Jan 1 2002
EventIEEE International Symposium on Biomedical Imaging, ISBI 2002 - Washington, United States
Duration: Jul 7 2002Jul 10 2002

Publication series

NameProceedings - International Symposium on Biomedical Imaging
Volume2002-January
ISSN (Print)1945-7928
ISSN (Electronic)1945-8452

Other

OtherIEEE International Symposium on Biomedical Imaging, ISBI 2002
CountryUnited States
CityWashington
Period07/7/0207/10/02

ASJC Scopus subject areas

  • Biomedical Engineering
  • Radiology Nuclear Medicine and imaging

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