A novel method of AquaporinZ incorporation via binary-lipid Langmuir monolayers

Guofei Sun, Hu Zhou, Yi Li, Kandiah Jeyaseelan, Arunmozhiarasi Armugam, Tai-Shung Chung*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

28 Scopus citations

Abstract

In this work, a new approach of incorporating transmembrane protein AquaporinZ (AqpZ) into a lipid bilayer has been developed with the aid of the Langmuir-Blodgett technique. The binary-lipid monolayer for AqpZ incorporation is composed of (1) gel-phase lipids resistant to detergent dissolution and (2) nickel-chelating lipids that can attach the histidine-tagged AqpZ from the subphase. Upon removal of subphase detergent with BioBeads, the incorporation is achieved by transferring the AqpZ-associated binary-lipid monolayer onto a preformed pure binary-lipid monolayer using the Langmuir-Schaefer deposition method. AFM images show an indication of AqpZ incorporation in the bilayer. Furthermore, it is also shown that BioBeads can remove a significant amount of detergent in the subphase and lipid film integrity is restored after detergent removal. The detergent removal rate is correlated to BioBeads amount and subphase circulation. The new approach of AqpZ reconstitution revealed in this work could potentially be applied in biomimetic membrane formation for water purification applications.

Original languageEnglish (US)
Pages (from-to)283-288
Number of pages6
JournalColloids and Surfaces B: Biointerfaces
Volume89
Issue number1
DOIs
StatePublished - Jan 1 2012

Keywords

  • Aquaporin
  • Detergent
  • Langmuir-Blodgett trough
  • Lipid monolayer
  • Protein incorporation

ASJC Scopus subject areas

  • Biotechnology
  • Surfaces and Interfaces
  • Physical and Theoretical Chemistry
  • Colloid and Surface Chemistry

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