A new equivalent circuit model for micro electroporation systems

Hooman Shagoshtasbi, Yi-Kuen Lee

Research output: Chapter in Book/Report/Conference proceedingConference contribution

5 Scopus citations

Abstract

Electroporation (EP) is a unique biotechnique in which intense electric pulses are applied on the cell membrane to temporarily generate nanoscale electropores and to increase the membrane permeability for the delivery of exogenous biomolecules or drugs. We propose a new equivalent circuit model with 8 electric components to predict the electrodynamic response of a micro EP system. As the permeability of the cell membrane increases, the membrane resistance decreases. The numerical simulations of the transmembrane current responses to different applied voltages (1∼6V) are consistent with the experimental results using HeLa cells. Besides, the transmembrane voltage as a function of applied voltages is determined as well. These transmembrane current and voltage responses can be extremely useful for the design of new generation of micro EP systems for transfection of large DNA molecules in the future. © 2011 IEEE.
Original languageEnglish (US)
Title of host publication2011 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems
PublisherInstitute of Electrical and Electronics Engineers (IEEE)
Pages662-665
Number of pages4
ISBN (Print)9781612847757
DOIs
StatePublished - Feb 2011
Externally publishedYes

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