A low rate of nucleotide changes in Escherichia coli K-12 estimated from a comparison of the genome sequences between two different substrains

Takeshi Itoh, Toshitsugu Okayama, Hiroyuki Hashimoto, Jun Ichi Takeda, Ronald W. Davis, Hirotada Mori, Takashi Gojobori*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

Two genome sequences of Escherichia coli K-12 substrains, one partial W3110 and one complete MG1655, have been determined by Japanese and American genome projects, respectively. In order to estimate the rate of nucleotide changes, we directly compared 2 Mb of the nucleotide sequences from these closely-related E. coli substrains. Given that the two substrains separated about 40 years ago, the rate of nucleotide changes was estimated to be less than 10-7 per site per year. This rate was supported by a further comparison between partial genome sequences of E. coli and Shigella flexneri. Copyright (C) 1999 Federation of European Biochemical Societies.

Original languageEnglish (US)
Pages (from-to)72-76
Number of pages5
JournalFEBS Letters
Volume450
Issue number1-2
DOIs
StatePublished - Apr 30 1999

Keywords

  • Complete genome
  • Escherichia coli
  • Genome project
  • Insertion sequence
  • Rate of nucleotide change
  • Rate of nucleotide substitution

ASJC Scopus subject areas

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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