A low-cost flow cytometric assay for the detection and quantification of apoptosis using an anionic halogenated fluorescein dye

Mervin Meyer*, Magbubah Essack, Stonard Kanyanda, Jasper G. Rees

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

We describe here a technical improvement of an established colorimetric method used to detect and measure the occurrence of apoptosis in mammalian cells during in vitro cell culture. This assay uses an anionic halogenated fluorescein dye that is taken up by apoptotic cells at the stage of phosphatidylserine externalization. We demonstrate that apoptotic cells stained with this dye can be detected by flow cytometric analysis. Furthermore, we show that the modified method compares well with the standard annexin-V-based apoptosis assay and that it is significantly more cost-effective than the annexin-V assay.

Original languageEnglish (US)
Pages (from-to)317-320
Number of pages4
JournalBioTechniques
Volume45
Issue number3
DOIs
StatePublished - Sep 1 2008

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry, Genetics and Molecular Biology(all)

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