A fluidic motherboard for multiplexed simultaneous and modular detection in microfluidic systems for biological application

Gerardo Perozziello*, Giuseppina Simone, Patrizio Candeloro, Francesco Gentile, Natalia Malara, Rosanna Larocca, Marialaura Coluccio, Salvatore Andrea Pullano, Luca Tirinato, Oliver Geschke, Enzo Di Fabrizio

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

We propose a polymer-based micro fluidic motherboard that integrates waveguides and fluidic networks providing an interface between micro fluidic systems and the outer world. The motherboard facilitates interconnections of several micro fluidic chips for multiplexed and simultaneous analysis. It offers a modular network for micro fluidic chips, allowing complex micro fluidic processes, where each microchip has a particular function. The motherboard includes micro fluidic channels machined by micro milling technology and bonded thermally. Waveguides were integrated in the motherboard in the same fabrication process. Additionally, pins were fabricated, which are part of the fluidic interconnections and allow the alignment of a chip with the waveguides of the motherboard. To establish high density micro fluidic interconnections between the motherboard and external tubes, reversible PDMS sockets have been utilized. For interconnecting the polymer waveguides to the light source and to the detection system, PDMS optical plugs have been developed. To demonstrate the performance of the device, test structures were designed that contained fluidic channels, waveguides and, additionally, custom made o-rings allowing alignment to the connection pins of the motherboard. The single chips were attached to the motherboard by just pressing them onto the alignment pins. A sealed fluidic connection between chip and motherboard is ensured by the deliberate mismatch in diameter between the o-rings of the chip and the pins of the motherboard. The maximally applicable hydraulic pressure before leakage, as well as propagation and coupling losses for the waveguides and optical interconnections have been characterized and tested.

Original languageEnglish (US)
Pages (from-to)227-238
Number of pages12
JournalMicro and Nanosystems
Volume2
Issue number4
DOIs
StatePublished - Jan 1 2010

Keywords

  • Interconnections
  • Interfacing
  • Microfluidic motherboard
  • Modular bio chemical analysis
  • Multiplexing

ASJC Scopus subject areas

  • Building and Construction

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