TY - JOUR
T1 - A donor-specific QTL, exhibiting allelic variation for leaf sheath hairiness in a nested association mapping population, is located on barley chromosome 4H
AU - Saade, Stephanie
AU - Kutlu, Burcu
AU - Draba, Vera
AU - Förster, Karin
AU - Schumann, Erika
AU - Tester, Mark A.
AU - Pillen, Klaus
AU - Maurer, Andreas
N1 - KAUST Repository Item: Exported on 2020-10-01
Acknowledgements: This project was funded by German Research Foundation (DFG) via Grants Pi339/7-1 and Pi339/8-1 (AM): http://www.dfg.de/; Interdisciplinary Centre for Crop Plant Research (IZN), Halle (VD): http://www.uni-halle.de/izn/; King Abdullah University of Science and Technology (KAUST) (SS): https://www.kaust.edu.sa/en; Ege University Erasmus Programme (BK): http://ebys.ege.edu.tr/ogrenci/ebp/llp-erasmus.htm.
PY - 2017/12/7
Y1 - 2017/12/7
N2 - Leaf sheath hairiness is a morphological trait associated with various advantages, including tolerance to both abiotic and biotic stresses, thereby increasing yield. Understanding the genetic basis of this trait in barley can therefore improve the agronomic performance of this economically important crop. We scored leaf sheath hairiness in a two-year field trial in 1,420 BC1S3 lines from the wild barley nested association mapping (NAM) population HEB-25. Leaf sheath hairiness segregated in six out of 25 families with the reference parent Barke being glabrous. We detected the major hairy leaf sheath locus Hs (syn. Hsh) on chromosome 4H (111.3 cM) with high precision. The effects of the locus varied across the six different wild barley donors, with donor of HEB family 11 conferring the highest score of leaf sheath hairiness. Due to the high mapping resolution present in HEB-25, we were able to discuss physically linked pentatricopeptide repeat genes and subtilisin-like proteases as potential candidate genes underlying this locus. In this study, we proved that HEB-25 provides an appropriate tool to further understand the genetic control of leaf sheath hairiness in barley. Furthermore, our work represents a perfect starting position to clone the gene responsible for the 4H locus observed.
AB - Leaf sheath hairiness is a morphological trait associated with various advantages, including tolerance to both abiotic and biotic stresses, thereby increasing yield. Understanding the genetic basis of this trait in barley can therefore improve the agronomic performance of this economically important crop. We scored leaf sheath hairiness in a two-year field trial in 1,420 BC1S3 lines from the wild barley nested association mapping (NAM) population HEB-25. Leaf sheath hairiness segregated in six out of 25 families with the reference parent Barke being glabrous. We detected the major hairy leaf sheath locus Hs (syn. Hsh) on chromosome 4H (111.3 cM) with high precision. The effects of the locus varied across the six different wild barley donors, with donor of HEB family 11 conferring the highest score of leaf sheath hairiness. Due to the high mapping resolution present in HEB-25, we were able to discuss physically linked pentatricopeptide repeat genes and subtilisin-like proteases as potential candidate genes underlying this locus. In this study, we proved that HEB-25 provides an appropriate tool to further understand the genetic control of leaf sheath hairiness in barley. Furthermore, our work represents a perfect starting position to clone the gene responsible for the 4H locus observed.
UR - http://hdl.handle.net/10754/626365
UR - http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0189446
UR - http://www.scopus.com/inward/record.url?scp=85037338429&partnerID=8YFLogxK
U2 - 10.1371/journal.pone.0189446
DO - 10.1371/journal.pone.0189446
M3 - Article
C2 - 29216333
AN - SCOPUS:85037338429
VL - 12
SP - e0189446
JO - PLOS ONE
JF - PLOS ONE
SN - 1932-6203
IS - 12
ER -