A DNA origami nanorobot controlled by nucleic acid hybridization

Emanuela Torelli, Monica Marini, Sabrina Palmano, Luca Piantanida, Cesare Polano, Alice Scarpellini, Marco Lazzarino, Giuseppe Firrao

Research output: Contribution to journalArticlepeer-review

38 Scopus citations

Abstract

A prototype for a DNA origami nanorobot is designed, produced, and tested. The cylindrical nanorobot (diameter of 14 nm and length of 48 nm) with a switchable flap, is able to respond to an external stimulus and reacts by a physical switch from a disarmed to an armed configuration able to deliver a cellular compatible message. In the tested design the robot weapon is a nucleic acid fully contained in the inner of the tube and linked to a single point of the internal face of the flap. Upon actuation the nanorobot moves the flap extracting the nucleic acid that assembles into a hemin/G-quadruplex horseradish peroxidase mimicking DNAzyme catalyzing a colorimetric reaction or chemiluminescence generation. The actuation switch is triggered by an external nucleic acid (target) that interacts with a complementary nucleic acid that is beard externally by the nanorobot (probe). Hybridization of probe and target produces a localized structural change that results in flap opening. The flap movement is studied on a two-dimensional prototype origami using Förster resonance energy transfer and is shown to be triggered by a variety of targets, including natural RNAs. The nanorobot has potential for in vivo biosensing and intelligent delivery of biological activators. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Original languageEnglish (US)
Pages (from-to)2918-2926
Number of pages9
JournalSmall
Volume10
Issue number14
DOIs
StatePublished - Mar 20 2014

ASJC Scopus subject areas

  • Medicine(all)

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