TY - JOUR
T1 - A Comprehensive Subcellular Atlas of the Toxoplasma Proteome via hyperLOPIT Provides Spatial Context for Protein Functions.
AU - Barylyuk, Konstantin
AU - Koreny, Ludek
AU - Ke, Huiling
AU - Butterworth, Simon
AU - Crook, Oliver M
AU - Lassadi, Imen
AU - Gupta, Vipul
AU - Tromer, Eelco
AU - Mourier, Tobias
AU - Stevens, Tim J
AU - Breckels, Lisa M
AU - Pain, Arnab
AU - Lilley, Kathryn S
AU - Waller, Ross F
N1 - KAUST Repository Item: Exported on 2020-10-19
Acknowledged KAUST grant number(s): BAS/1/1020-01-01, OSR-2015-CRG4-2610
Acknowledgements: This work was supported by the Medical Research Council, United Kingdom, MR/M011690/1 to R.F.W.; King Abdullah University of Science and Technology (KAUST), Saudi Arabia, OSR-2015-CRG4-2610 to A.P., R.F.W., and K.S.L.; Wellcome Trust, United Kingdom, Investigator award 214298/Z/18/Z to R.F.W.; an Isaac Newton Trust-Leverhulme, Early Career Fellowship ECF-2015-562 to K.B; and KAUST faculty baseline funding (BAS/1/1020-01-01) to A.P. Mass spectrometry data were acquired by Mike Deery at the Cambridge Centre of Proteomics, and we thank Laurent Gatto for useful discussions.
PY - 2020/10/14
Y1 - 2020/10/14
N2 - Apicomplexan parasites cause major human disease and food insecurity. They owe their considerable success to highly specialized cell compartments and structures. These adaptations drive their recognition, nondestructive penetration, and elaborate reengineering of the host's cells to promote their growth, dissemination, and the countering of host defenses. The evolution of unique apicomplexan cellular compartments is concomitant with vast proteomic novelty. Consequently, half of apicomplexan proteins are unique and uncharacterized. Here, we determine the steady-state subcellular location of thousands of proteins simultaneously within the globally prevalent apicomplexan parasite Toxoplasma gondii. This provides unprecedented comprehensive molecular definition of these unicellular eukaryotes and their specialized compartments, and these data reveal the spatial organizations of protein expression and function, adaptation to hosts, and the underlying evolutionary trajectories of these pathogens.
AB - Apicomplexan parasites cause major human disease and food insecurity. They owe their considerable success to highly specialized cell compartments and structures. These adaptations drive their recognition, nondestructive penetration, and elaborate reengineering of the host's cells to promote their growth, dissemination, and the countering of host defenses. The evolution of unique apicomplexan cellular compartments is concomitant with vast proteomic novelty. Consequently, half of apicomplexan proteins are unique and uncharacterized. Here, we determine the steady-state subcellular location of thousands of proteins simultaneously within the globally prevalent apicomplexan parasite Toxoplasma gondii. This provides unprecedented comprehensive molecular definition of these unicellular eukaryotes and their specialized compartments, and these data reveal the spatial organizations of protein expression and function, adaptation to hosts, and the underlying evolutionary trajectories of these pathogens.
UR - http://hdl.handle.net/10754/665619
UR - https://linkinghub.elsevier.com/retrieve/pii/S193131282030514X
U2 - 10.1016/j.chom.2020.09.011
DO - 10.1016/j.chom.2020.09.011
M3 - Article
C2 - 33053376
JO - Cell Host and Microbe
JF - Cell Host and Microbe
SN - 1931-3128
ER -
