A comparative study of electrostatic repulsion-hydrophilic interaction chromatography (ERLIC) versus SCX-IMAC-based methods for phosphopeptide isolation/enrichment

Chee Sian Gan, Tiannan Guo, Huoming Zhang, Sal Kiang Lim, Siu Kwan Sze

    Research output: Contribution to journalArticlepeer-review

    87 Scopus citations

    Abstract

    Electrostatic repulsion-hydrophilic interaction chromatography (ERLIC) has been introduced recently for phosphopeptide enrichment. Here we compared ERLIC with the well-established SCX-IMAC for identifying phosphopeptides in EGF-treated A431 cells. The ERLIC approach detected a higher number of phosphopeptides (17 311) than SCX-IMAC (4850), but it only detected 926 unique phosphopeptides compared to 1315 in SCX-IMAC. Only 12% unique phosphopeptides were common to both approaches, suggesting that more comprehensive phosphoproteomes could be generated by complementing SCX-IMAC with ERLIC.

    Original languageEnglish (US)
    Pages (from-to)4869-4877
    Number of pages9
    JournalJournal of Proteome Research
    Volume7
    Issue number11
    DOIs
    StatePublished - Nov 1 2008

    Keywords

    • A431 carcinoma cells
    • EGFR signaling
    • ERLIC
    • IMAC
    • Phosphopeptide enrichment
    • SCX
    • WAX

    ASJC Scopus subject areas

    • Biochemistry
    • Chemistry(all)

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