A classic cAMP responsive element in the promoter region of the α1- GABA(A) receptor subunit has non-classic properties

Franz M. Gerner, Thorsten Trapp, Charlotte A E Hauser*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

The cAMP responsive element binding protein (CREB) and the glucocorticoid receptor (GR) have been reported to bind to a 60 bp promoter fragment of the α1-GABA(A) receptor gene containing a classic cAMP- responsive element (CRE). We inserted this fragment into a hormone responsive element-deleted mouse mammary tumor virus promoter controlling the expression of luciferase. Activation of GR showed no significant change in luciferase expression, but hormone induction by forskolin revealed a reduction in neuronal cell lines. Furthermore, we demonstrate that cellular factors from neuronal cells can bind to the CRE-containing promoter fragment, although competition by unlabeled CRE and GRE oligo-nucleotides is not present. Mutation of the CRE site and deletion of neighboring DNA sequences indicate that the promoter is probably associated with a complex of different regulatory factors.

Original languageEnglish (US)
Pages (from-to)55-61
Number of pages7
JournalFunctional Neurology
Volume12
Issue number2
StatePublished - Mar 1997
Externally publishedYes

Keywords

  • α subunit
  • CREB
  • GABA(A) receptor
  • glucocorticoid receptor
  • promoter

ASJC Scopus subject areas

  • Clinical Neurology
  • Neuroscience(all)

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