A Chemically Well-Defined, Self-Assembling 3D Substrate for Long Term Culture of Human Pluripotent Stem Cells

Yihua Loo, Yun S. Chan, Iwona Szczerbinska, Bobby C. P. Tan, Andrew C.A Wan, Huck Hui Ng, Charlotte Hauser

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Clinical applications of human pluripotent stem cells (PSCs) are limited by the lack of chemically well-defined scaffolds for cell expansion, differentiation and implantation. In this study, we systematically screened various self-assembling hexapeptides to identify the best matrix for long-term 3D PSC culture. Lysine-containing Ac-ILVAGK-NH2 hydrogels maintained best the pluripotency of human embryonic and induced PSCs even after 30 passages. This peptide matrix is also compatible with the use of xeno-free and defined differentiation media. By exploiting its stimuli-responsive sol-gel transition, arrays of encapsulated PSCs can be bioprinted for large-scale cell expansion and derivation of miniaturized organoid cultures for high-throughput screening.
Original languageEnglish (US)
Pages (from-to)1406-1412
Number of pages7
JournalACS Applied Bio Materials
Volume2
Issue number4
DOIs
StatePublished - Mar 2019

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