3D imaging of surfaces and cells by numerical reconstruction of wavefronts in digital holography applied to transmission and reflection microscopy

Tristan Colomb, Etienne Cuche, Pia Dahlgren, Anca M. Mariana, Frederic Montfort, Christian Depeursinge*, Pierre Marquet, Pierre Magistretti

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

4 Scopus citations

Abstract

We report on a numerical method for the reconstruction of wavetonts in Digital Holographic Microscopy (DHM). It yields simultaneous amplitude and quantitative phase contrast imaging by acquiring a single hologram. The reconstruction method computes the propagation of the complex optical wavefront diffracted by the object and is used to determine the refractive index and/or shape of the object with accuracies in the nanometer range along the optical axis. The technique comprises the recording of a digital hologram of the specimen by means of a standard CCD camera at the exit of a Mach-Zender or Michelson type interferometer and an adjustment procedure to determine precisely the reconstruction parameters. The quantitative nature of the reconstructed phase distribution has been demonstrated by an application to surface profilometry where step height differences of a few nanometers have been measured. Another application takes place in biology for transmission phase-contrast imaging of living cells in culture with resolution reaching 30 nanometers in height.

Original languageEnglish (US)
Title of host publication2002 IEEE International Symposium on Biomedical Imaging, ISBI 2002 - Proceedings
PublisherIEEE Computer Society
Pages773-776
Number of pages4
ISBN (Electronic)078037584X
DOIs
StatePublished - Jan 1 2002
EventIEEE International Symposium on Biomedical Imaging, ISBI 2002 - Washington, United States
Duration: Jul 7 2002Jul 10 2002

Publication series

NameProceedings - International Symposium on Biomedical Imaging
Volume2002-January
ISSN (Print)1945-7928
ISSN (Electronic)1945-8452

Other

OtherIEEE International Symposium on Biomedical Imaging, ISBI 2002
CountryUnited States
CityWashington
Period07/7/0207/10/02

ASJC Scopus subject areas

  • Biomedical Engineering
  • Radiology Nuclear Medicine and imaging

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