16S-23S rRNA internal transcribed spacers as molecular markers for the species of the 16S rRNA group I of the genus Bacillus

Daniele Daffonchio*, Sara Borin, Arianna Consolandi, Diego Mora, Pier Luigi Manachini, Claudia Sorlini

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

55 Scopus citations

Abstract

The internal transcribed spacers between the 16S and the 23S ribosomal RNA genes were used to discriminate species of the 16S rRNA group 1 of the genus Bacillus by PCR. The spacer-PCR fingerprints clearly discriminated the different species, except those closely related like the members of the 'B. cereus group' (B. cereus, B. thuringiensis and B. mycoides) and the species of the 'B. subtilis group' (B. amyloliquefaciens and B. licheniformis). Examining in more detail the shortest internal transcribed spacers, B. subtilis group species were distinguished by single-strand conformation polymorphism analysis, whereas B. mycoides was differentiated from B. cereus/B. thuringiensis by restriction analysis.

Original languageEnglish (US)
Pages (from-to)229-236
Number of pages8
JournalFEMS Microbiology Letters
Volume163
Issue number2
DOIs
StatePublished - Jun 15 1998

Keywords

  • 16S rRNA group 1
  • Bacillus
  • Identification
  • Internal transcribed spacer- polymerase chain reaction fingerprinting
  • Restriction analysis
  • Single-strand conformation polymorphism

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Microbiology

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